| Overview |
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| Microarray data of S. typhimurium |
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For the identification of the PhoPQ regulon in S. typhimurium, we set up a microarray experiment in which RNA was isolated from strains of Salmonella enterica serovar Typhimurium ATCC 14028s harbouring either a null mutation (phoP::Tn10dCm) or constitutive mutation (phoQ24) in the genes encoding the PhoPQ two-component system (Miller and Mekalanos 1990). Strains were grown to mid-log phase in M9 minimal media prior to harvesting RNA and the array experiments were performed as described in Bader et al. (2003). Data were statistically analysed using the maximum-likelihood analysis of Ideker et al. (2000, 2001). These data can be found here.
| PhoPQ regulated genes that are differentially expressed in S. typhimurium as well as E. coli |
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A detailed list of PhoPQ regulated genes that are differentially expressed in S. typhimurium as well as E. coli, and information about the presence of the PhoP motif and the function of the genes, can be found here.
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| Differentially expressed PhoPQ regulated genes in S. typhimurium but not in E. coli |
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A detailed list of PhoPQ regulated operons that are differentially expressed in S. typhimurium but not in E. coli with information about the expression and function of the individual genes that belong to thes operons, can be found here.
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| Differentially expressed PhoPQ regulated genes in E. coli but not in S. typhimurium |
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A detailed list of PhoPQ regulated operons that are differentially expressed in E. coli but not in S. typhimurium with information about the expression and function of the individual genes that belong to thes operons, can be found here.
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| References |
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Bader MW, Navarre WW, Shiau W, Nikaido H, Frye JG, McClelland M, Fang FC , Miller SI (2003) Regulation of Salmonella typhimurium virulence gene expression by cationic antimicrobial peptides. Mol Microbiol 50:219-230
Ideker T, Galitski T, Hood L (2001) A new approach to decoding life: systems biology. Annu Rev Genomics Hum Genet 2:343-372
Ideker T, Thorsson V, Siegel AF, Hood LE (2000) Testing for differentially-expressed genes by maximum-likelihood analysis of microarray data. J Comput Biol 7:805-817
Miller SI, Mekalanos JJ (1990) Constitutive expression of the phoP regulon attenuates Salmonella virulence and survival within macrophages. J Bacteriol 172:2485-2490