BlockSampler Additional Datasets

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Six additional datasets were included in the analysis: ERG3, GSH1, HIV-EP1, HOXB5, Meis2 and PCDH8. Each dataset consisted of different combinations of mammals chicken, Fugu, Tetraodon and zebrafish. The compositions of the initial datasets can be found here.
ERG3 is a developmental regulator (zinc finger protein), which is most likely involved in muscle spindle development. For this gene, two conserved blocks were found in mammals and fish.
GSH1 has an important function in pituitary development. This data set contained four blocks that are conserved among human, chimp, mouse, rat, and pufferfish (Fugu and Tetraodon).
HOXB5 is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Six strongly conserved blocks were detected in HOXB5 orthologs of mammals and pufferfish, while the motif seems to have been lost in chicken.
HIV-EP1 is a zinc finger protein that specifically binds to enhancer elements present in several viral promoters and a number of cellular promoters such as those of the class I MHC, interleukin-2 receptor, and interferon-beta genes. No significant blocks were detected in this non-developmental gene.
Meis2 is expressed in various tissues such as the lymphoid organs and some regions of the brain. For Meis2 two blocks were recovered that are retained in all organisms under study, except for Fugu.
The protocadherin 8 precursor PCDH8 is believed to function as a calcium-dependent cell-adhesion protein. In this non developmental related data set one large block was detected, conserved in human, chimp, mouse, rat, chicken, Tetraodon and Fugu, but not in zebrafish.

gene	blockID	consensus	organisms
EGR3	block 1.1	TGnCnCGCnGCCCynCGACCCTCCCnnCA	human, chimp, mouse, dog, Tetraodon, zebrafish
	block 1.2	TTGTCTGTCCATATATGGnCAnCTACGTCAC	human, chimp, mouse, dog, Tetraodon, zebrafish
GSH1	block 1.1	TnTTnCGGCGTGGGTGGGGnTGACAAGAATAGAnTACATTATGCAGTTCATTTAGTTAACAAGTGAAATAATGnGGAAGCGTGCAGnGnGAATGCCnAGAGAA	human, chimp, mouse, rat, Fugu, Tetraodon
	block 1.2	TTAGTTAACAAGTGAAATAATGnGGAAGCGTGCAGnGnGAATGCCnAGAGAAAnGnnnnAAAnnCnnTnnnG	human, chimp, mouse, rat, Fugu, Tetraodon
	block 1.3	AAAACCCTATTGAGAGnnnnnGGCCGCTnnnnGCGTAnn	human, chimp, mouse, rat, Fugu, Tetraodon
	block 1.4	AAAnTGAAAGAAAATGTTTTCCTATTACTTAATTCAATCAnAG	human, chimp, mouse, rat, Fugu, Tetraodon
HOXB5	block 1.1	GTCATnATTTGTAACCATAGAGCATGAATTACCTCTTGAnGTCATCAGnGAGAATTTACGACTGGTCAACAAAnGCACGTGAT	human, chimp, mouse, rat, Tetraodon, zebrafish
	block 1.2	CnCCCATATTTGGCCGCATACATAGCAAA	human, chimp, mouse, rat, Tetraodon, zebrafish
	block 1.3	TAATTCATTAATACATCATAAATCGTGAAGCACAGGGTTATAACGACCAnGATCnACAAATCAAGCCCTCnAAAA	human, chimp, mouse, rat, Tetraodon, zebrafish
	block 1.4	AAACGAAGTACAGTGCATnGCTATAATTCATTAATACATCATAAATCGTGAAG	human, chimp, mouse, rat, Tetraodon, zebrafish
	block 1.5	AAACGAAGTACAGTGCATnGCTATAATTCATTAATACATCATA	human, chimp, mouse, rat, Tetraodon, zebrafish
	block 1.6	ACGAAGTACAGTGCATnGCTATAATTCATTAATACATCATAAATCGTGA	human, chimp, mouse, rat, Tetraodon, zebrafish
MEIS2	block 1.1	TAACCCCAAAATGACCCCAATTTGACACCrCAAGATGAAATTTTACCGCCTGTTAAAACCA	human, chimp, mouse, rat, chicken, Tetraodon, zebrafish
	block 1.2	ATGAAATTTTACCGCCTGTTAAAACCATTTCCAGCCTGGGCnn	human, chimp, mouse, rat, chicken, Tetraodon, zebrafish
PCDH8	block 1.1	CTAACGAGGGCTTCATAAGCCTTTGATACAGTCTGATCTTTGAAAC	human, chimp, mouse, rat, chicken, Fugu, Tetraodon

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